《植物生理学报》 2014, 50(10): 1445-1452

非损伤微测技术在植物生理学研究中的应用及进展

李静, 韩庆庆, 段丽婕, 王沛, 李惠茹, 王锁民, 张金林*

兰州大学草地农业科技学院, 草地农业生态系统国家重点实验室, 兰州730020

通信作者：张金林；E-mail: jlzhang@lzu.edu.cn；Tel: 0931-8913447

摘　要：

非损伤微测技术(NMT)是一种选择性微电极技术, 可以在不损伤活体样品的情况下获得进出样品的各种离子或分子浓度、流速及其三维运动方向的信息。该技术具有非损伤性、长时间、多电极、高分辨率、高灵敏度、多角度测量等优点, 可获得其他技术难以测到的生理特征和生命活动规律, 从而在理论研究和应用领域产生实质性的突破。本文综述了近年来NMT在植物营养调控、抗病性、抗盐碱、抗旱、抗冷、抗重金属毒害等方面的应用及其进展, 并展望了NMT在植物生理学研究中的应用前景。

关键词：非损伤微测技术; 植物营养调控; 生物胁迫; 非生物胁迫

收稿：2014-05-04   修定：2014-08-26

资助：国家自然科学基金项目(31222053、31170431和31172256)、教育部长江学者和创新团队发展计划(IRT13019)、新世纪优秀人才支持计划(NCET-11-0217)、中央高校基本科研业务费项目(lzujbky-2013-k08和lzujbky-2013-bt05)。

Applications and Advances of Non-Invasive Micro-Test Technique in Plant Physiology Researches

LI Jing, HAN Qing-Qing, Duan Li-Jie, WANG Pei, LI Hui-Ru, WANG Suo-Min, ZHANG Jin-Lin*

State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Corresponding author: ZHANG Jin-Lin; E-mail: jlzhang@lzu.edu.cn; Tel: 0931-8913447

Abstract:

Non-invasive micro-test technique (NMT) is a kind of selective microelectrode technique which can get the information on concentration, flux rate and three-dimensional movement of ions or molecules going in or out of the samples without any damages. The technique has the advantages of being non-invasive, long-term, multi-electrodes, high resolution, high sensitivity and multi-angle measurement etc. NMT can aid scientists to obtain physiological characteristics and life activity rules of the tested samples that are difficult to be detected by other techniques. In this paper, the application and development of NMT in plant nutrition regulation, disease resistance, and salt, alkali, drought, cold and heavy metal tolerance etc. are reviewed. Finally, the application of NMT in plant physiology is prospected.

Key words: non-invasive micro-test technique; plant nutrition regulation; biotic stress; abiotic stress